From the data calculate the concentrations for the two most abundant species in the unknown histidine solution (ie. Finally, let's summarize It also provides a list of pKa values of buffers commonly used in biology and biochemistry. Click here. NMR can yield structural fingerprints for a protein biologic at atomic resolution that are intrinsically dependent on higher order structure. pH of this buffer solution represented in the particulate diagram. of the acetate anion. So for a generic weak acid, we could call that HA, and therefore, its 0000003286 00000 n Thinking about the However, the price might be considered a drawback, as well as the tendency of histidine to interact with metal ions. This page titled 7.24: Calculating pH of Buffer Solutions- Henderson-Hasselbalch equation is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Ed Vitz, John W. Moore, Justin Shorb, Xavier Prat-Resina, Tim Wendorff, & Adam Hahn. WebSolution for Prepare 1.0 L of a 0.1 M histidine buffer, pH 7.0, starting with histidine dihydrochloride (C3H11N3O2Cl2, FW 228), 0.5 N NaOH, and water. No data are ever sent to the molbiotools.com server. divided by the concentration of the weak acid. In preparation of the material for public availability, many methods were qualified for their intended use in assessing the identity (e.g., peptide mapping), purity (e.g., capillary zone electrophoresis [CZE]), monomeric purity (size exclusion chromatography [SEC] and capillary sodium dodecylsulfate electrophoresis [CE-SDS]), and stability (dependent on attributes) of the NISTmAb. I like to take wildlife photographs with a little narrative [, One of my long term interests has been in protein turnover - the process whereby proteins are synthesised and degraded inside the cell, sometimes at remarkably high rates. 0000050237 00000 n Glycine at pH=7.0 is shown below: Step 1. Normally a good buffer should not interact with other components. Lock So for this buffer solution, the pH would be greater than 4.74. A complete glycation profile was determined, for the first time, for all possible glycation sites in the NISTmAb. Results will be published in a peer reviewed journal. In 2020, an interlaboratory study of glycosylation profiles of a reference and modified IgG antibody involving 103 reports from 76 laboratories was reported by Stephen Stein and Lorna A De Leoz et al., in Mol Cell Proteomics. An interlaboratory study of the glycosylation of a reference antibody: NISTmAb. 7. To log in and use all the features of Khan Academy, please enable JavaScript in your browser. Buffer Calculations: Formula and Equations 1 Molar solution equation: desired molarity formula weight solution final volume (L) = grams needed 2 Percentage by weight (w/v): (% buffer desired / 100) final buffer volume (mL) = g of starting material needed 3 Henderson-Hasselbach equation: pH = pKa + log [A-]/ [HA] Find the pH of the solution obtained when 1.00 mol NH3 and 0.40 mol NH4Cl are mixed to give 1 L of solution. Simply enter whatever electrolytes you are adding, then hit calculate below. WebPrepare 20mM citrate buffer at pH 5.5 using HendersonHasselbalch equation? I took a chance on a print run of 500 sets, and I'm pleased to say that there are only about 50 sets left. A buffer will only be able to soak up so much before being overwhelmed. Since the hydronium-ion concentration is governed by, \[[\text{H}_{3}\text{O}^{+}]=K_{a}\frac{[\text{CH}_{3}\text{COOH}]}{[\text{CH}_{3}\text{COO}^{-}]}\]. So the pH is equal to the pKa, which we calculated in 2020 Jan;19(1):11-30. The same amount of acetate ion will be produced. 1.7-2.9 . The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. buffer 1.2-2.6 . Direct link to bob ross's post hi there, may i know what, Posted 9 months ago. If you're seeing this message, it means we're having trouble loading external resources on our website. Analysis involved two samples, the NISTmAb and an enzymatically modified sample, enabling within-lab separation of random and systematic errors using the Youden two-sample method. of the buffer solution was equal to the pKa of the weak acid. 0000041679 00000 n particle in the solution, but to give us an idea hb```"7Abl,'d@nxfTRp/+Wv1Y`03d^X%%]4rK"bN%-:ep{\VoP5crM .mqvlDGG sXCDgmVag "F eo;. This paper, published at the beginning of 2023, is [. Input buffer volume, concentrated multiple, pH to get formula. A highly complex glycosylation profile was achieved for the NISTmAb, including 60 unique glycan compositions, almost more than double earlier reports for any individual mAbs expressed in CHO, NS0, and other cells. But, my, Made a decision in 2021 to harvest the sun! WebHistidine has 3 pKas of 1.54, 6.07, and 9.34. And in the third example, the concentration of the weak acid was less than the concentration Remember that the goal acid and its conjugate base, the acetate anion. of the buffer solution is less than the pKa of the weak acid. approximate and only valid for diluted solutions (< 100mM) and in the pH range of pK. A vial of RM 8671 contains 800 L of 10 mg/mL IgG1 monoclonal antibody in 12.5 mmol/L L-histidine, 12.5 mmol/L L-histidine HCl (pH 6.0). A lock ( of this buffer solution. Details [. ] A single 1 L container of 14HB was diluted 10-fold and aliquoted as RM 8671 lot 14HB-D-001. of the acetate anion. Fragments from various peptide, glycopeptides, and disulfide-linked peptides in all three libraries are fully annotated. So whenever the concentration Click here to see all available distributors, PBS (Phosphate Buffered Saline) (1X, pH 7.4), BES-Buffered Saline (2X) (0.05 M, pH 6.95), Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6), Citrate-Phosphate Buffer (0.15 M, pH 5.0), Citrate-Phosphate Buffer (110 mM, pH 5.6), EBSS (magnesium, calcium, phenol red) (pH 7.0), Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10), Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0), Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix, Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes, Sodium Carbonate Transfer Buffer (40x, pH 9.5). Therefore, the pH of the buffer solution was greater than the pKa of the weak acid. Buffer Range alphabetically. Let's look at another particulate diagram. Share sensitive information only on official, secure websites. 0000000976 00000 n The development of the three NISTmAb mass reference spectral libraries provides comprehensive data of tryptic peptides and their various biological modifications required to support industrys need in determining the properties of mAbs with high-degree heterogeneity. The effective buffering range of a buffer is between 1 of the maximal buffering capacity. Thus, the effective buffering range of histidine is pH 5.12 to pH 7.12 and pH 8.45 to pH 10.45. If NaOH has not been accurately prepared, method used in (c) (i) will give a more reliable estimate of the pKa values. The NISTmAb material is a recombinant humanized IgG1 expressed in murine suspension culture. Fixed typos. Ads help to keep molbiotools up, running and evolving. conjugate base would be A-. WebL-Histidine has a molecular weight of 155.15 g/mol and its pH in an aqueous solution is 7 - 8.5 Solution 2%. (Hemoglobin, a protein, is the red substance in the blood. ElISA Kits For Food Safety & Drug Residues, Recombinant Antibodies for Drug Discovery, Lead Selection of Antibody Drug Discovery, reconstitution/ molarity/dilution calculator, Native Chromatin Immunoprecipitation(ChIP), Cross-linking Chromatin Immunoprecipitation (ChIP), Reconstitution (Concentration) Calculator / Molarity / Dilution Calculator, A (Monopotassium Phosphate, MW: 136.09 g/mol), A (Disodium Hydrogen Phosphate, MW: 141.96 g/mol), B (Sodium Dihydrogen Phosphate, MW: 119.98 g/mol), A (Potassium Hydrogen Phthalate, MW: 204.23 g/mol). These and other product quality attributes were extensively characterized in the ACS book series "State of the Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization" for the initial batch of material (interim material 8670), used as the Primary Standard (PS) herein. This wide range is due to phosphoric acid having 3 dissociation constants, (known in chemistry as a triprotic acid) allowing for formulation of buffers near each of the pH levels of 2.15, 6.86, or 12.32. Adjust solution to final desired pH using HCl or NaOH. qTotal = i = 1, nfHAqHA + fA qA walkthrough Calculate the net charge on the amino acid glycine at pH 2.0. So we can go back to the WebHistidine buffer can be used for anion exchange columns, having about the same buffer range as piperazine. Approximate pH values are calculated with DATA PRIVACY: All user data input into the apps are processed locally within Let's count the number of Any suggestions are warmly welcome. Input buffer volume, molar concentration, pH to get formula. In this case, the Forced degradation studies were performed in order to further elucidate potential degradation pathways and production of product-related impurities relevant for challenging methods during qualification exercises. 0000041567 00000 n 0000004693 00000 n To support it effectively, please click the ads only if you have at least a potential interest in the product and. We are frequently asked questions on the use of buffers that we offer to research laboratories. Number of moles of HCl Then, following the formula, we divide n by the change in pH of the sodium phosphate solution. Note: Ensure enough feed material and appropriate system working volume in Let us now consider the general problem of finding the pH of a buffer solution which is a mixture of a weak acid HA, of stoichiometric concentration ca, and its conjugate base A, of stoichiom, \[[\text{H}_{3}\text{O}^{+}]=K_{a}\times \frac{[\text{HA}]}{[\text{A}^{-}]}\label{6}\], Taking negative logarithms of both sides, we obtain, \[-\text{log }[\text{H}_{3}\text{O}^{+}]=-\text{log }K_{a}-\text{log}\frac{[\text{HA}]}{[\text{A}^{-}]}\], \[\text{pH}=\text{p}K_{a}\text{+ log}\frac{[\text{A}^{-}]}{[\text{HA}]}\label{8}\]. during a session (it makes all ad clicks invalid), thank you! WebThe buffer calculator can calculate various buffers which used to do experiment, including PBS Buffer, Acetic Acid-Sodium Acetate Buffer, Barbitone Sodium-HCl Buffer, Barbiturate To calculate the pH of It is grounded in quality measurements, thus providing a common control material for originator and follow on manufacturers alike. *Significant deviations exist in the reported values of pKa and ) or https:// means youve safely connected to the .gov website. Utilization of Biodegradable Hydroponic Growth Media as a Carbon Source for Greenhouse Wastewater Denitrification, Lipase in oat endosperm: The effect of freeze-drying and oven-drying, Potential Enhancement of Metformin Hydrochloride in Solidified Reverse Micellar Solution-Based PEGylated Lipid Nanoparticles Targeting Therapeutic Efficacy in Diabetes Treatment, Biotranformation Of Environmental Toxicants By Different Enzymes, Click here to see all available distributors, Change the value in the textbox above to scale the recipe volume, Phosphate Buffer (pH 5.8 to 7.4) Preparation and Recipe, PBS (Phosphate Buffered Saline) (1X, pH 7.4), BES-Buffered Saline (2X) (0.05 M, pH 6.95), Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6), Citrate-Phosphate Buffer (0.15 M, pH 5.0), Citrate-Phosphate Buffer (110 mM, pH 5.6), EBSS (magnesium, calcium, phenol red) (pH 7.0), Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10), Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0), Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix, Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes, Sodium Carbonate Transfer Buffer (40x, pH 9.5), https://www.aatbio.com/resources/buffer-preparations-and-recipes/phosphate-buffer-ph-5-8-to-7-4, Adjust the molarity of the solution by using the slider below, Adjust the pH of the solution by using the slider below, Adjust solution to final desired pH using HCl or NaOH. Jan '23: Made a start on a general reorganisation of this web site. At 25 degrees Celsius, the Calculate the pH of an acetate buffer that contains 0.0300 M CH3COOH and 0.0400 M CH3COO-. Methods The hydrodynamic radius of the mAb was determined by dynamic light acid-acetate buffer solution. The most important of these is undoubtedly the H2CO3/HCO3 pair, but side chains of the amino acid histidine in the hemoglobin molecule also play a part. of the conjugate base, the pH of the buffer solution is equal to the pKa of the weak acid. And therefore, the pH Henderson-Hasselbalch equation to think about the relative concentrations of the weak acid and the conjugate base. Histidine buffer has a concentration of 0.1M and a pH of 6.0. In the first example, the concentration of the weak acid was equal to the concentration The RM is intended for a variety of uses that may include system suitability tests, establishing method or instrument performance and variability, comparing changing analytical test methods, and assisting in method qualification. Since we have more acetic acid particles than acetate particles, the concentration of acetic acid is greater than the concentration of the conjugate base. Critical quality attributes (CQA) are significant measurement parameters of a medical product that impact both product safety and efficacy and are essential characteristics that are linked to positive public health outcomes. ACS Book series: "State-of-the-Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization", Volume 1 - Monoclonal Antibody Therapeutics: Structure, and Regulatory Space, The NISTmAb Reference Mass Spectral Libraries and Related Publications. The molecule also has N-terminal pyroglutamination, C-terminal lysine clipping, and glycosylation of the heavy chains. If the same 0.5 mol had been added to a cubic decimeter of pure water, the pH would have jumped all the way from 7.00 up to 13.7! 2. the side effects which vary with the tissue type: a. The primary goal of the NMR interlaboratory project is to use the Fab domain from the NISTmAb to demonstrate the robustness of the NMR measurement and to validate NMR structural fingerprinting measurements for the assessment of higher order structure of large protein biologics and/or domains from these proteins. Buffers consists of a weak trailer <<07B480EF654B42749C43AD60C8AA854F>]/Prev 150825/XRefStm 1497>> startxref 0 %%EOF 397 0 obj <>stream ads based on a user's prior visits to this website or other websites. 364 34 0000001907 00000 n 0000007773 00000 n Histidine Buffer And the log of one is equal to zero. A basic buffer solution is simply one where the pH > 7. When [HA] = [A], the solution pH is equal to the pK of the acid. Histidine buffer has a concentration of 0.1M and a pH of 6.0. DISCLAIMER: This free software comes without any Henderson-Hasselbalch equation is an equation that's often used to calculate the pH of buffer solutions. To get a basic pH we just need to adjust the concentrations of the acid and conjugate base correctly. Webb-mercaptoethanol (20 l to 980 l sample buffer) before use. And so first, we need to know Additional characterization assays of dynamic light scattering and flow imaging analysis of protein particulates were also employed. Wehopethis material finds widespread utility in the biomanufacturing community. pH of the buffer solution. there are only four. Osmolarity Calculator - is that concentration too high?? A locked padlock The NISTmAb is also serving as the current basis for advancing measurement techniques at NIST such as small angle neutron scattering, nuclear magnetic resonance, x-ray diffraction crystallography, small angle X-ray scattering, mass spectrometry multi-attribute method, and glycan and peptide mass spectral libraries, to name a few. Since the hydronium-ion concentration is so small, very little hydroxide ion will be consumed by reaction with the hydronium ion. Considering about it, there is a sweet guy in my company developing this buffer calculator online so that you have no worries on buffer calculating. We still have an acetic The buffer is one of the most popular currently used, and is commonly employed in molecular and cell biology, chemistry, and material science, among many others. WebBuffer Calculator is an online tool for buffer pH calculations. 0000026779 00000 n 0000001497 00000 n Henderson-Hasselbalch equation to think about the pH pKa 25C . 0000002978 00000 n less than one is negative. %PDF-1.7 % WebCalculate the overall charge by summing the contribution of each group using the following formula. Fill all but one field and click 'Calculate': WARNING: Calculations Henderson-Hasselbalch equation and write that the pH is equal to the pKa, which we just calculated to be 4.74 plus the log of the concentration warranty. Contributors utilized the NISTmAb throughout, demonstrated the potential utility of class-specific reference materials as a means to facilitate open innovation, and identified a number of emerging research areas for future development. The voluntary and open access nature of this material makes it the premier choice for technology development in the pre-competitive space. Practice Problems acid and its conjugate base. Even though most products pages of antibodies, proteins or other reagents will list the formula, changes are everywhere. 364 0 obj <> endobj xref A spectral library-based novel workflow for complete disulfide mapping of the nine NISTmAb native SS bonds as well as 86 SS bonds arising from experiment artifacts. In tabular form: Substituting the equilibrium concentrations of base (acetate ion) and conjugate acid (acetic acid) into the Henderson-Hasselbalch equation, Eq. So let's count our particles. It can save your time on the process of preparing experiment. The buffer capacity will be best near each pKa. It also provides a list of pKa values of buffers commonly used in biology and biochemistry. WebJavascript is required. effective pH range . Dear researchers, we know you must have lots of work to do for your research. To find the pKa of acetic acid, we take the negative log of the Ka value. WebHistidine Buffer Protonation States The simulations were performed at an l -histidine (L-HIS) buffer concentration of 20 mM, which is a typical concentration used in mAb 0000001679 00000 n of the acetate anion is greater than the So if we know the pH of a buffer solution, we can think about the WebThis question deals with the concepts of buffer capacity and buffer range. The material was produced in murine suspension cell culture and has undergone industry standard upstream and downstream purification to remove process related impurities. Once again, we count our particles. of the acetate anion. Therefore, we can say 103 reports were received from 76 diverse laboratories worldwide. the log of the concentration of the conjugate base Use the contact form if any electrolytes are not present that you need. Henderson-Hasselbalch equation. Therefore, we would be 0000003748 00000 n Legal. Added new pages relevant to the Amino Acid Card Game. Made small changes to phbuffer web pages, but no changes to design algorithms. 0000009166 00000 n The NIST monoclonal antibody reference material is, quite possibly, the most widely characterized publicly available monoclonal antibody, a molecule directly relevant to the biopharmaceutical industry. These molecules embody various complex attributes, the characterization of which is a long and arduous process, yet monoclonal antibody therapeutics have taken residence as perhaps one of the most influential therapeutic classes of our time. 0000004041 00000 n WebOur histidine buffers, available with a pH of 6.0 and 7.0, are ideal for buffering amino acid solutions. To support it effectively, please click the ads only if you have at least a potential interest in the product and do not click them repeatedly Official websites use .gov 0000008039 00000 n So we would be subtracting We look forward to industry feedback on the technical utility of NISTmAb RM 8671 as well as the suitability of related follow on materials that may supplement this robust and critical class of therapeutic. Adjust solution to final desired pH using HCl or NaOH Add distilled water until the volume is 1 L. of the conjugate base. Identify ionizable groups, their pKa values, and their charges. the previous problem for acetic acid, it's 4.74 at 25 degrees Celsius, plus the log of the concentration So fundamentally it's no different from the buffer system shown in this video. WebThe hydrodynamic radius initially increased with increasing histidine concentration, going through a maximum at a histidine concentration of about 20 mM. the particulate diagrams of buffer solutions, water molecules and cations An official website of the United States government. 0000002903 00000 n particular buffer solution and we know the pH of the buffer solution is less than the pKa of the weak acid, we know that in that buffer Approximate pH values are concentration of acetic acid is greater than the concentration The protein has low abundance post-translational modifications including methionine oxidation, deamidation, and glycation. WebSpecial cases: Histidine, proline, glycine, cysteine Amino acid structure Isoelectric point and zwitterions Classification of amino acids Four levels of protein structure Conformational stability: Protein folding and denaturation The structure and function of globular proteins Test prep > MCAT > Foundation 1: Biomolecules > Amino acids and proteins The buffer calculator can calculate various buffers which used to do experiment, including PBS Buffer, Acetic Acid-Sodium Acetate Buffer, Barbitone Sodium-HCl Buffer, Barbiturate Buffer, Borax-NaOH Buffer, Phosphate Buffer, Barbiturate Buffer, Glycine-HCl Buffer, Tris-HCl Buffer, KH2PO4-NaOH Buffer, et al. are often left out for clarity. This booklet is designed to help answer basic questions about the use of buffers in biological systems. NISTmAb Mass Spectral Library of Human IgG1 mAb Drugs, Disulfide-Linked (SS) Peptides Spectral Library, https://chemdata.nist.gov/dokuwiki/doku.php?id=peptidew:mab, NISTmAb Interlaboratory Study on Glycosylation Analysis. Description The NISTmAb material is a recombinant humanized IgG1 expressed in murine suspension culture. will go virtually to completion, and 0.50 mol acetic acid will be consumed. We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. Paper [, A new paper with our colleagues led by Simon Hubbard in Manchester showing that is possible to aid in the selection and assembly of peptides for QconCAT design or ALACAT assemblies. An updated version has a few additional amino acid solutions that were requested as well as improved printing. For example, if we have a How many buffer regions does histidine have? is the acetate anions, so let's write that in here, CH3COO-, and that's divided by the If you're behind a web filter, please make sure that the domains *.kastatic.org and *.kasandbox.org are unblocked. of the conjugate base. It is responsible for carrying oxygen away from the lungs.) WebBioworld Histidine Buffer 0.1M, pH 6.0 | 500mL. WebCommon preparation methods include: 1) dripping an acid (or alkali) into an aqueous solution of a salt while measuring the pH with a pH meter and 2) making an aqueous solution of acid with the same concentration as the salt and mixing while measuring the pH with a pH meter. adding a number to 4.74. WebFinal buffer Copt = 110/2.71828 = 40.5 g/L The Cg/e method can only be used when the flux vs. concentration data allows for accurate extrapolation to zero flux. So for acetic acid, this Manufacturing Extension Partnership (MEP), The NIST monoclonal antibody(NISTmAb)reference material, Volume 2 - Biopharmaceutical Characterization: The NISTmAb Case Study, Volume 3 - Defining the Next Generation of Analytical and Biophysical Techniques, Mol Cell Proteomics. the pH of the solution would be less than 4.74. WebPublish a Booklet on Buffers? WebHistidine Buffer Calculator - Wakelet masdeajettoo @masdeajettoo926 Follow 3 items Histidine Buffer Calculator Buffering Region of Histidine Monohydrochloride - 2726 time, there are four particles and for the acetate anion, this time, there are six particles. The objective of this study was to examine the effects of both histidine and sucrose on the biophysical characteristics of a mAb. And therefore, the pH We can use the In order to use Eq. Molbiotools is a collection of free online apps: A free online tool for buffer pH calculations. Phosphate buffer is highly water soluble and has a high buffering capacity, but will inhibit enzymatic activity and precipitates in ethanol. Let's use the We analysed over 3000 samples, and built models that could predict species, sex and most importantly, the age of the mosquito - the number of oviparous cycles is related to the number of blood meals a female take, and blood meals mean malaria. Most enzymes (biological catalysts) can only function inside a rather limited pH range and must therefore operate in a buffered environment. Histidine has a pKa of 6.2 but this can range from 5 8 when the amino acid is bound to other amino acids. Molar Solutions desired To calculate the amount of buffer needed, please select the desired buffer from the The buffer is extremely effective at resisting a change in pH because the added hydroxide ion attacks the weak acid (in very high concentration) rather than the hydronium ion (in very low concentration). Input buffer volume, concentrated multiple to get formula. Therefore, all of this would maleate (pK1) . of a particulate diagram is not to represent every All rights reserved. 1.97 . Therefore, the ratio Thus, \[\begin{align}\left[\text{H}_{3}\text{O}^{+}\right]=K_{a}\times \frac{c_{a}}{c_{b}}\\\text{ }=\text{5.56}\times \text{ 10}^{-10}\text{ mol L}^{-1}\times \frac{\text{ 0.4 mol L}^{-1}}{\text{1.0 mol L}^{-1}}\\\text{ }=\text{2.22 }\times \text{ 10}^{-10}\text{ mol L}^{-1}\end{align}\], To see why a mixture of an acid and its conjugate base is resistant to a change in pH, let us go back to our first example: a mixture of acetic acid (3 mol L1)and sodium acetate (2 mol L1). WebBuffer Calculator Using the buffer preparation calculator This buffer calculator provides an easy-to-use tool to calculate buffer molarity and prepare buffer solutions using the L-Histidine HCl has a molecular weight of 209.63g/mol. concentration of acetic acid. 0000001871 00000 n Add 20.214 g of Sodium Phosphate Dibasic Heptahydrate to the solution. Thus, the buffer capacity of our sodium phosphate solution is 0.14. In addition, we have launched three research tools in succession, involving reconstitution/ molarity/dilution calculator, molecular weight calculator and ELISA data analysis.
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